Advanced HPLC Method Development

A Short Course

By

John Dolan, PhD

LC Resources Inc.

Place:                   MiddleBrook Pharmaceuticals

                    20425 Seneca Meadows Parkway

                   Germantown, MD 20876

Date:          July 24-25, 2008

Time:                    Registration 8:30AM

                   Course 9:00AM

Cost:           Early Registration (before July 11): $450/person

                   Late Registration: $500/person

Space is limited so please reserve your spot with Alan Potts (apotts@middlebrookpharma.com; 301-944-6601)!!

Payment must be made with check or money order to Washington Chromatography Discussion Group or WCDG.  No shows will be charged the full amount of $500.

Advanced HPLC Method Development    

Abstract:

This 1.5 day class is designed for laboratory workers involved in HPLC method development as well as those who must transfer of existing methods into a routine laboratory. The class focuses on separation fundamentals and applies them in a time-proven strategy for developing robust HPLC methods quickly. Based on the instructor’s extensive experience in a laboratory supporting pharmaceutical and bioanalytical methods, the attendees should gain practical skills to develop realistic HPLC methods in a short time. The techniques can be used as a stand-alone strategy or added to existing development procedures to help streamline the process. The course content assumes a basic understanding of HPLC, but not necessarily experience in method development.  Students will be introduced to HPLC method development software to help find satisfactory separation conditions. Attendees will receive a comprehensive set of course notes, including copies of all visual materials presented.

Biographical Sketch:

John Dolan is a partner in LC Resources, a company dedicated to training professionals in HPLC and related techniques.  John received his Ph.D. from the University of California at Davis in 1976 and did postdoctoral work at Northeastern University. He worked at Technicon Instruments Corporation and IBM Instruments prior to starting LC Resources in 1984. LCR grew to provide training, software, and laboratory services in HPLC. The laboratory and software segments were sold in 2002, at which time John remained as General Manager for the BASi Northwest Laboratory through 2005. John has written the monthly LC Troubleshooting column in LC/GC magazine since 1983.  His research interests have generated over 100 scientific papers on HPLC theory, instrumentation, and applications as well as a book on troubleshooting HPLC instruments and methods and one on gradient elution (both with Lloyd Snyder). He has taught short courses on HPLC for LC Resources and the American Chemical Society to more than 10,000 students over the last 20 years.

Course Content

Day 1 – 9:00AM to 4PM

Section 1 – Introduction to HPLC Method Development

Section 2 – HPLC Basics

Section 3 – HPLC Columns

Section 4 – Reversed-Phase Separation

Section 5 – Dealing with Ionic Samples

A light breakfast and a lunch will be provided.

Day 2 – 9:00AM to 12PM

Section 6 – Ion Pair Chromatography

Section 7 – Gradient Elution

Section 8 – Normal Phase and HILIC

A light breakfast will be provided.