Washington Chromatography Discussion Group
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FEATURING DR. STEVEN M. MUSSER
Center for Food Safety and Applied Nutrition
Food and Drug Administration
College Park, MD
DATE THURSDAY, MARCH 18, 2004
TIME 6:00 p.m. Informal Dinner
7:00 p.m. Presentation
LOCATION U.S. PHARMACOPEIA
12701 Twinbrook Parkway, Rockville, MD 20852
ABSTRACT
LC/MS Method Development for the Analysis of Dietary Supplements
Steven M. Musser, Ph.D., Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD 20740
Botanical dietary supplements have a long history of use as alternatives to Western medical approaches for the treatment of disease. In part, this can be attributed to the widely held belief that these products are inherently safe because they are derived from natural sources. Nevertheless, dozens of life-threatening adverse events are reported each year which can be attributed to consumption of botanical dietary supplements. Since the passage of the Dietary Supplement Health and Education Act of 1994 (DSHEA), FDA and in particular, the Center for Food Safety and Applied Nutrition, has been tasked with determining the safety of these products. One of the critical components of research associated with DSHEA is the development of analytical methods. These methods are needed for the accurate determination of active constituents, measuring potential contaminants and assessing label claims. To meet these needs, liquid chromatography/mass spectrometry (LC/MS) is often chosen as the methodology of choice. Although LC/MS offers a number of advantages in terms of sensitivity, selectivity, and overall speed of analysis, there are a number of considerations which must be kept in mind when developing analytical methods that rely on this technology. Some of these critical components include choice of internal standards, column availability and appropriate ionization mode. The impact of these critical components on method development will be discussed using examples of well known dietary supplements.
BIOGRAPHICAL SKETCH
Dr. Musser is Chief of the Instrumentation and Biophysics Branch and Lead Scientist for Chemistry at the FDA Center for Food Safety and Applied Nutrition. He received his Ph.D. in Medicinal Chemistry from the University of Maryland, Baltimore, MD and prior to joining the FDA worked as a post-doctoral fellow at the National Cancer Institute of the National Institutes of Health. Dr. Musser has numerous scientific publications, and is the author of several book chapters related to the analysis of food. He has received several patents and regularly teaches courses on mass spectrometry and analytical chemistry at scientific meetings and graduate schools. He is currently responsible for research projects in each of the following four areas.
1) Instrument based quantitative and confirmatory assays for nutritional supplements and natural toxins in food. 2) Natural toxin metabolite and biomarker screening methods using liquid chromatography/mass spectrometry. 3) Identification and complete structure determination of new natural toxins. 4) Development and evaluation of new instrument based methods for the analysis of allergens and microorganisms.
WCDG 2003-2004 CALENDAR
September 18, 2003 -- Meeting cancelled due to Hurricane Isabel
October 16, 2003 -- J. Faye Rubinson, Georgetown University
"Conducting Polymer Electrodes as Detectors for HPLC and FIA"
November 20, 2003 -- Curtis S. Phinney, National Institute of Standards and Technology
December 11, 2003, -- John G. Dorsey, Florida State University
"Reversed Phase Liquid Chromatography: Partitioning Processes at Interfaces"
January 15, 2004 -- Robert Q. Thompson, Oberlin College
"Silver Bells and Habaneros: Polishing Capsaicinoid Chromatography"
February 19, 2004 -- Luis Colon, University at Buffalo, State University of New York
"Exploring the limits of particles size in HPLC and CEC"
March 18, 2004 -- Steven Musser, Center for Food Safety and Applied Nutrition, Food and Drug Administration
"LC/MS Method Development for the Analysis of Dietary Supplements"
April 15, 2004 -- Ronald Manning, United States Pharmacopeia
May 20, 2004 -- David Bell, Supelco
June 24, 2004 -- Proposed Forensics Roundtable
and accepting nominations and voting for 2004-2005 officers during this meeting
POSTER PRESENTATIONS
If you are interested in displaying a poster during the social hour (6-7pm), contact Mary Satterfield or Kate Rimmer.
WCDG CALL FOR VOLUNTEER SPEAKERS, SUGGESTED TOPICS, SPEAKERS
Please contact a WCDG Program Chair:
Mary Satterfield, 301-975-5364, mary.satterfield@nist.gov
Kate Rimmer, 301-975-3651, catherine.rimmer@nist.gov
2003-2004 WCDG OFFICERS
President Dittmar Wulff dittmar@dcsci.com
Co-Program Mary Satterfield mary.satterfield@nist.gov
Chairs/VP Kate Rimmer catherine.rimmer@nist.gov
Secretary Reenie Parris reenie.parris@nist.gov
Treasurer Patricia White paw@usp.org
WCDG Office Janet Cunningham janetbarr@aol.com
MEMBERSHIP DUES
Please send dues of $10 to: WCDG, P.O. Box 279, Walkersville, MD 21793. Your membership is very important as it will help us obtain company sponsorships to keep this program going.
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