Miniaturized Liquid Separations: CE and Bioanalysis

Lisa Holland, C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV 26506

Low volume liquid based separations present clear advantages in bioanalysis. The simplicity of the approaches, the flexibility and ease in altering separation parameters, rapid method development, small sampling volume, higher separation efficiency, as well as potential fieldability, portability and affordability of devices and methodology have resulted in the diverse applications of CE. Separation based assays, affinity screening, partitioning, and binding interaction will be demonstrated with standard and non-standard CE additives. The capabilities of CE for both constituent analysis as well as the elucidation of significant thermodynamic or kinetic information will be demonstrated in the context of protein, peptide, and small molecule analyses.

Lisa Holland Biography:

Lisa Holland received her BS degree in Chemistry from the University of Maryland at College Park in 1990, while working in the Electroanalytical Research Group at the National Institute of Standards and Technology. She received her Ph.D. in Chemistry from the University of North Carolina at Chapel Hill in 1996, under the direction of Professor James Jorgenson. Through a National Research Service Award, she held a postdoctoral fellowship, under the direction of Professor Susan Lunte in the Department of Pharmaceutical Chemistry at the University of Kansas. She currently holds a faculty position in the C. Eugene Bennett Department of Chemistry at West Virginia University, specializing in bioanalytical separations. Dr. Holland is the recipient of a National Science Foundation Faculty Early Career Development award, has served on the scientific committee of the Frederick Conference on Capillary Electrophoresis, and has numerous publications in the field of separations chemistry.